碳酸锂对抗AFB1诱导大鼠肝癌过程中MDA、4-HNE的表达及意义

doi:10.3969/j.issn.1004-616x.2005.03.002

癌变·畸变·突变 ›› 2005, Vol. 17 ›› Issue (3): 133-135.doi: 10.3969/j.issn.1004-616x.2005.03.002

碳酸锂对抗AFB1诱导大鼠肝癌过程中MDA、4-HNE的表达及意义

黄晓欣1/张爱华2,＊/洪　峰2/陆　爽2/岑笃才

中国人民解放军第44医院，贵州　贵阳 550009

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-05-30 发布日期:2005-05-30

Expression of MDA and 4-HNE in AFB1-Induced Rat Hepatic Putative reneoplastic Lesions Alleviated by Li2CO3 and Their Significance

HUANG Xiao-xin1， ZHANG Ai-hua2, HONG Feng2 LU Shuang2， CEN Du-cai1

44th Hospital of PLA，Guiyang 550009,Guizhou,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-05-30 Published:2005-05-30

摘要/Abstract

摘要： 背景与目的：采用免疫组化法检测碳酸锂(Li2CO3)对抗黄曲霉素B1(Aflatoxin B1,AFB1)诱导大鼠肝癌过程中丙二醛(Malondialdehyde,MDA)、4-羟壬烯醛(4-hydroxynonenal,4-HNE)的表达情况，探讨Li2CO3的抗癌作用及其机制。材料与方法： 144只Wistar大鼠随机分为A、B、C、D 4组，分别为阴性、阳性对照及Li2CO3同时给药和Li2CO3先期给药组。于实验第6、9、10周分批断头处死动物，取动物的肝脏进行肝组织形态学检查及免疫组织化学染色。结果： C、D两组动物健康状况明显改善，肝癌前病变程度明显减轻； MDA、4-HNE在诱癌早期(实验第6周)即有表达，第10周显著增高，并呈B组>C组>D组>A组的趋势。结论： Li2CO3具有明显地对抗和抑制化学诱导肝癌过程中的脂质过氧化(Lipid peroxidation,LPO)作用；免疫组化法检测MDA、4-HNE可敏感而特异的反映机体氧化损伤程度，有助于肝癌的早期发现及病变进展的动态观察。

关键词: 丙二醛, 4-羟壬烯醛, 碳酸锂, 大鼠肝脏, 癌前病变

Abstract: BACKGROUND ＆ AIM: In order to observe the anti-carcinoma use of lithium carbonate(Li2CO3) and it's mechanism, the histopathological change and the expression of malondialdehyde (MDA) and 4-hydroxynonenal(4-HNE)protein in Aflatoxin B1 (AFB1)-induced rat hepatic putative preneoplastic lesions alleviated by lithium carbonate(Li2CO3). MATERIAL AND METHODS: One hundred and forty-four healthy Wistar rats (155～175 g) were divided randomly into four groups: normal control group (group A), positive control group (group B), group treated with Li2CO3 simultaneously (group C), group pre-treated with Li2CO3 (group D). The rats were killed in batches in the 6th, 9th or 10th week of the experiment. Histopathological and immunohistochemical assay of MDA and 4-HNE protein was made on hepatic. RESULTS: The condition of rats was significantly improved in Group C and Group D, so do the severity of preneoplasm. Coinciding with the histopathological discoveries, a tendency of "Group B>Group C>Group D>Group A" could be found in observation of MDA and 4-HNE protein expressive, which occurred in the early stage (the 6th week) and significantly increased in the 10 th week. CONCLUSION: It suggested that Li2CO3 has obvious against or restrain lipid peroxidation effect on hepatoma induced by chemicals. The immunohistochemistry assay of MDA and 4-HNE can be used as a sensitive and specialize method to reflect oxidative damage，and it may help early finding and monitoring of hepatoma.

Key words: malondialdehyde, 4-hydroxynonenal, Li2CO3, rat hepatic, preneoplastic lesion

黄晓欣/张爱华, ＊/洪　峰/陆　爽/岑笃才. 碳酸锂对抗AFB1诱导大鼠肝癌过程中MDA、4-HNE的表达及意义[J]. 癌变·畸变·突变, 2005, 17(3): 133-135.

HUANG Xiao-xin, ZHANG Ai-hua, HONG Feng LU Shuang, CEN Du-cai. Expression of MDA and 4-HNE in AFB1-Induced Rat Hepatic Putative reneoplastic Lesions Alleviated by Li2CO3 and Their Significance[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(3): 133-135.

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碳酸锂对抗AFB1诱导大鼠肝癌过程中MDA、4-HNE的表达及意义

Expression of MDA and 4-HNE in AFB1-Induced Rat Hepatic Putative reneoplastic Lesions Alleviated by Li2CO3 and Their Significance

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